Application

Flow Cytometry (1-5 µg/ml)r>Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1-2 µg/sample)r>Immunoblotting (not recommended)

General description

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor protein.

This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1).

Immunogen

K562 cells

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Schneider, C., et al., 1982. J. Biol. Chem.257, 8516.Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem.49, 357.Omary, M.B., et al. 1980. Nature286, 888.Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.

Packaging

100 µg in Plastic ampoule

Reconstitution

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)